Title : Reveal Ralstonia eutrophus as a carrier of poly-β-hydrobutyrate for the culture of slipper lobster (Thenus orientalis) larvae
Abstract :Application of poly-β-hydroxybutyrate (PHB) supplemented in a bacterial carrier (i.e., amorphous PHB) was shown to work positively for the culture of Macrobrachium rosenbergii larvae, Litopenaeus vannamei and Penaeus monodon postlarvae. In this study, PHB accumlating Ralstonia eutrophus was investigated as a biocontrol agent for disease in adult Artemia (9 days old) and Thenus orientalis larvae culture. The application of amorphous PHB at the tested concentrations was shown to significantly protect adult Artemia, as well as T. orientalis larvae when challenged with V. harveyi at 5x108 cells mL-1. Furthermore, feeding phyllosoma larvae with amorphous PHB enriched in adult Artemia completely inhibited the growth of Vibrio spp. in the gut of larvae. In conclusion, PHB supplemented in a bacterial carrier through the Artemia could be increased the survival, growth and disease protection of slipper lobster larvae when challenged with pathogenic V. harveyi.