Rotifers are important live nourishment for larval fish and shellfish in aquaculture. In the present examination, we directed a trial to assess the appropriateness of Chlorella ellipsoidea as nourishment for generation of the rotifer, Brachionus calyciflorus. The examination was completed in three medications utilizing dried powder Chlorella ellipsoidea (T1), crisp live Chlorella ellipsoidea (T2) and Bread cook's yeast (T3) as nourishment with three replications. For providing nourishment to rotifers, C. ellipsoidea was refined adequately in various cheap culture media before rotifer culture and it was proceeded up to the finish of the rotifer culture try. The scopes of natural components dissected were appropriate for both C. ellipsoidea and rotifers culture during the trial time frame. Greatest cell densities of C. ellipsoidea were recorded in cheap heartbeat wheat remove medium during the way of life period. The mean populace densities (implies ± SEM) of B. calyciflorus were essentially (p<0.01) most elevated in T2 (28.6 ± 4.64 (x103) people L-1) contrasted with T1 (11.6 ± 1.24 (x103) people L-1) and T3 (11.4 ± 1.82 (x103) people L-1). The discoveries of present investigation uncovered that crisp refined C. ellipsoidea was the best nourishment for creation of the rotifer, B. calyciflorus.