Title : Invitro activity of natural antimicrobials against Multidrug resistant microorganisms isolated from pus samples

The utilization of both plant extracts and phytochemicals, with remarkable antimicrobial properties, can be of great importance for therapeutic applications, particularly in the treatment of diseases caused by Multidrug resistant microorganisms. The frame work of the current study was mainly designed to study the efficacy of some naturally existing plant derived, fungal derived and algal derived antimicrobial substances against the multidrug resistant microorganisms isolated from diabetic foot lesions of the patients. The pus samples collected from diabetic foot lesion patients in a Tertiary care hospital were processed immediately in the Microbiology laboratory for the isolation and identification of pathogens through morphological, cultural and biochemical characterization. Antibiotic susceptibility pattern of isolated pathogens was checked by Kirby Bauer disc diffusion method using Clinical and Laboratory Standards Institute (CLSI) guidelines. Different naturally available compounds like Honey, spices, spirulina and mushroom were used to determine their antibacterial activity against the wound isolates through agar-well diffusion method at varying test concentrations against Staphylococcus aureus, Klebsiella pneumoniae, E. coli, Pseudomonas aeruginosa, Proteus mirabilis, Coagulase negative staphylococci, Enterococcus faecalis, E. coli 25922 and S.aureus 25927. The results obtained in the current study propose that bioactive and structurally diverse fungal metabolite i.e. mushroom extracts, herbal products like spices showed mild antibacterial activity while to honey and spirulina at highest concentrations. The study helps in the development of valuable pharmaceutical products and may be one of the better options for treating the infections caused by multidrug resistant bacterial isolates