This study aimed to explore attitudes and preferences towards non-invasive prenatal testing (NITP) among nursing students and students with other majors and to identify factors influencing their attitudes and preferences. This study was conducted with 382 college students in Korea. Collected data were analyzed using descriptive analyses, t-test for quantitative data and a content analysis for the open-ended questions. More than half of the nursing students reported that they had heard about NIPT while only a small number of their counterparts did. Nursing students were more positive about NIPT than their counterparts and more likely to indicate that their beliefs played a role in determining if they would choose to undergo NIPT. Findings from this study suggest there is a need to educate college students, especially nursing students about NIPT because they need to be prepared to provide culturally and socially sensitive care to patients and their families.