Journal ID : ALS-17-09-2020-5511
[Volume - 10, Issue - 04]
Total View : 578
Title : DAZ Gene Deletion Occurs in Indonesian Infertile Men with Abnormal Sperm Regardless of Ethnic Background
Abstract :

The purpose of this study is analyzing the DAZ gene deletion in infertile men oligozoospermia to azoospermia on Javanese and non-Javanese ethnics in Indonesia and whether the occurrence of deletion is affected by ethnic. A hundred sample from infertile men consisting of 45 Javanese and 55 non-Javanese ethnics were used. The sperm were selected and categorized based on the results of semen analysis which includes volume and pH, concentration, motility, and morphology. The genom DNA was extracted from peripheral blood and analyzed for DAZ gene deletion. Analysis of deletion for each ethnic used descriptive approach while the effect of deletion on the ethnic used Chi-square statistic. The results showed that 5 samples (11%) had DAZ gene deletion in Javanese and 7 samples (13%) in nonJavanese ethnic. Based on the category of sperm abnormalities, 5 samples with DAZ gene deletion in Javanese ethnic consisted of 4 and 1 samples with azoospermia and severe oligospermia group, respectively. In non-Javanese ethnic, 3 samples are from azoospermia group and 4 samples are from severe oligozoospermia group. None deletion occurred in the oligozoospermia group from both ethnics. Both ethnics had 50% asthenozoospermia, meanwhile, 16 samples (62%) from the Javanese and 12 samples (43%) from the non-Javanese had teratozoospermia. It indicated that the DAZ gene deletion affected spermatogenesis to produce the quantity and quality of sperm. Also, the deletion occurrence was randomly occured and regardless the ethnic background

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