Asia Life Sciences

Abstract : Diabetes mellitus and Hypetension are the gateways to cardiovascular diseases and they never hesitate to conquer any community or population. Patients are at high risk of cardiovascular disease when Diabetes Mellitus and Hypertension coexist, which requires accurate risk assessment and focused treatment. Measurement of Apolipoprotein- B and Non -HDL cholesterol as part of a routine lipid profile could play a pivotal role in optimal atherosclerotic risk reduction. Hence, we studied the relationship between Apolipoprotein-B, Non -HDL cholesterol and lipid profiles in diabetic patients with Hypertension. Apolipoprotein-B, Lipid Profile and Non-HDL cholesterol were estimated in Type 2 Diabetic patients with hypertension and they were compared with age and sex-matched healthy controls. Serum was analyzed for lipid profile in a fully automated Beckman AU 480 analyzer, Non-HDL cholesterol was calculated value and Serum Apo-B by the turbidimetry method was done in a semi autoanalyzer. Significant increase in values of Apolipoprotein-B, fasting blood sugar, total cholesterol, LDL- cholesterol, Triglycerides, and Non-HDL cholesterol was seen in type 2 diabetic patients with Hypertension compared to controls. There was a significant positive correlation between Apolipoprotein-B and fasting blood sugar, total cholesterol, LDL-cholesterol, triglycerides, and Non-HDL cholesterol. The findings of the present study stated that the majority of the patients studied had low HDL-C, elevated non-HDL-C, elevated total cholesterol, high triglycerides, elevated LDL-C, and elevated Apolipoprotein-B. Apolipoprotein-B had a positive linear correlation with total cholesterol, triglycerides, LDL-C, non-HDL-C. The strongest positive correlation was with non-HDL-C. Patients with low HDL-C had high Apo B levels

Abstract : The purpose of this study is analyzing the DAZ gene deletion in infertile men oligozoospermia to azoospermia on Javanese and non-Javanese ethnics in Indonesia and whether the occurrence of deletion is affected by ethnic. A hundred sample from infertile men consisting of 45 Javanese and 55 non-Javanese ethnics were used. The sperm were selected and categorized based on the results of semen analysis which includes volume and pH, concentration, motility, and morphology. The genom DNA was extracted from peripheral blood and analyzed for DAZ gene deletion. Analysis of deletion for each ethnic used descriptive approach while the effect of deletion on the ethnic used Chi-square statistic. The results showed that 5 samples (11%) had DAZ gene deletion in Javanese and 7 samples (13%) in nonJavanese ethnic. Based on the category of sperm abnormalities, 5 samples with DAZ gene deletion in Javanese ethnic consisted of 4 and 1 samples with azoospermia and severe oligospermia group, respectively. In non-Javanese ethnic, 3 samples are from azoospermia group and 4 samples are from severe oligozoospermia group. None deletion occurred in the oligozoospermia group from both ethnics. Both ethnics had 50% asthenozoospermia, meanwhile, 16 samples (62%) from the Javanese and 12 samples (43%) from the non-Javanese had teratozoospermia. It indicated that the DAZ gene deletion affected spermatogenesis to produce the quantity and quality of sperm. Also, the deletion occurrence was randomly occured and regardless the ethnic background