Asia Life Sciences

It is known that morphological and anatomical analysis is an important method for identifying medicinal plant materials (MPR). To date, not all types of pharmacopoeial plants have an optimal description of microscopic diagnostic signs. This article presents the study of the morpho-anatomical structure of the vegetative and generative organs of the medicinal, alkaloid and essential oil plant Haplophyllum perforatum, growing in the conditions of Uzbekistan, and identifies characteristic diagnostic signs, and also determines the localization of biologically active substances. The revealed features are species-specific diagnostic signs and can be used as additional diagnostic signs for the identification of species and plant materials

The aim of this study was to determine the appropriate dosage of NPK fertilizer (16-16-8) for the growth of Dunaliella sp. Two separate experiments were conducted in a controlled temperature (24 ± 2.0 °C) room. Microalgae were inoculated in 8 L jars at 25‰, and initial cell density was inoculated at 1 × 106 cells/mL. In the first experiment, NPK (N:P ratio of 1:1) was added to microalgae culture medium, with N concentrations of 8, 16, and 24 mg/L. The results showed that the highest cell density was obtained with 4.7 × 106 cells/mL on day 11 with the 16 mg/L treatment. In the second study, microalgae were cultured with different ratios of N:P—from 1:1 to 15:1—in which the final N concentration in the culture medium was 16 mg/L. The results showed that the cell density was highest at 5.72 × 106 cells/mL in the N:P treatment ratio of 9:1 on day 9, and the dry weight of biomass reached 0.15 g/L on day 7. Overall, the results of this study indicated that NPK can be used to culture Dunaliella sp., but it is necessary to adjust the N:P ratio to 9:1 in order to obtain the highest growth of the cell population