Asia Life Sciences

A two-factorial experimental design was used to evaluate the combined effect of three stocking densities—500, 750, and 1,000 ind./L—and three C/N ratios—5, 10, and 15—on the growth and biomass production of Artemia franciscana. Artemia nauplii (newly hatched) were reared in 1.5 L bottles containing 1 L of sea water at 30‰. Molasses with a 38% carbon content was used as carbon source to stimulate bacterial growth in the Artemia culture medium. Results showed that the manipulation of C/N at 5 and 10 promoted better growth of Artemia in terms of length compared to that obtained by Artemia in the higher C/N ratio. However, it was not clear whether there was an increase of growth performance in the culture where Artemia were reared at high densities. Moreover, the addition of molasses produced a biomass in stocking density at 500 ind./L that was nearly similar to that obtained in the 750 and 1,000 ind./L

Containing high-quality RNA is the basis of plant molecular biology research, plant genetics and other physiological investigations. At present, several plant RNA isolation methods have been utilized to received high-quality RNA yield. However, there are not much research data regarding RNA resistance after RNA isolation stage. This study attempts to elaborate on the effects of RNA storage on RNA quality for general molecular biology experiments such as Reverse Transcriptase PCR to obtain specific DNA fragments. Temperature and concentration were chosen because they are the most basic factors that can affect RNA quality. The samples were checked using Nanodrop spectrophotometer and gel electrophoresis for testing purity, quantity and integrity of RNA. Based on this experiment, total RNA from sugarcane can be stored at -40 °C and -20 °C for a month without significant quality quantity reduction. Both storage temperatures allow the quality of RNA to be maintained at a sufficient level for use in cDNA synthesis and gene-specific amplification without additional treatment. Storing pure RNA at a sufficiently high concentration would be more advantageous for the next experimental stage